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DNA Modifying Enzymes & Cloning Technologies

NEB offers a comprehensive list of tools and reagents for DNA manipulation. These include ligases, nucleases, methyltransferases, DNA repair proteins, recombinases and more.

NEBcloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow.

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DNA Modifying Enzymes and Cloning Technologies

Nucleoside Digestion Mix

Argonautes

Tth Argonaute (TtAgo)

DNA Ligases

9°N™ DNA Ligase

Blunt/TA Ligase Master Mix

E. coli DNA Ligase

ElectroLigase®

HiFi Taq DNA Ligase

Hi-T4™ DNA Ligase

Immobilized T4 DNA Ligase

Instant Sticky-end Ligase Master Mix

NEBNext® Quick Ligation Module

NEBridge™ Ligase Master Mix

Quick Blunting™ and Quick Ligation™ Kits

Quick Ligation™ Kit

Salt-T4® DNA Ligase

SplintR® Ligase

StickTogether™ DNA Ligase Buffer

T3 DNA Ligase

T4 DNA Ligase

T4 DNA Ligase Reaction Buffer

T7 DNA Ligase

Taq DNA Ligase

Taq DNA Ligase Reaction Buffer

Thermostable 5´ App DNA/RNA Ligase

DNA Repair Enzymes and Structure-specific Endonucleases

Afu Uracil-DNA Glycosylase (UDG)

Antarctic Thermolabile UDG

APE 1

Endonuclease III (Nth)

Endonuclease IV

Endonuclease V

Endonuclease VIII

EnGen® Mutation Detection Kit

Fpg

hAAG

hSMUG1

Mismatch Endonuclease I

PreCR® Repair Mix

RecA

RecAf

T4 PDG (T4 Endonuclease V)

T7 Endonuclease I

Thermolabile USER® II Enzyme

Thermostable FEN1

Tma Endonuclease III

Topoisomerase I (E. coli)

Tth Endonuclease IV

Uracil Glycosylase Inhibitor (UGI)

Uracil-DNA Glycosylase (UDG)

USER® Enzyme

Methyltransferases

AluI Methyltransferase

BamHI Methyltransferase

CpG Methyltransferase (M.SssI)

dam Methyltransferase

EcoGII Methyltransferase

EcoRI Methyltransferase

GpC Methyltransferase (M.CviPI)

HaeIII Methyltransferase

Hhal Methyltransferase

HpaII Methyltransferase

Human DNA (cytosine-5) Methyltransferase (Dnmt1)

MspI Methyltransferase

TaqI Methyltransferase

Exonucleases and Non-specific Endonucleases

DNase I (RNase-free)

DNase I Reaction Buffer

Exonuclease I (E. coli)

Exonuclease I Reaction Buffer

Exonuclease III (E. coli)

Exonuclease T

Exonuclease V (RecBCD)

Exonuclease VII

Exonuclease VIII, truncated

Lambda Exonuclease

Micrococcal Nuclease

Mismatch Endonuclease I

Mung Bean Nuclease

Nuclease P1

RecJf

T5 Exonuclease

T7 Endonuclease I

T7 Exonuclease

Thermolabile Exonuclease I

Phosphatases & Sulfurylases

Antarctic Phosphatase

Antarctic Phosphatase Reaction Buffer

Apyrase

Pyrophosphatase, Inorganic (E. coli)

Pyrophosphatase, Inorganic (yeast)

Quick CIP

Shrimp Alkaline Phosphatase (rSAP)

Thermostable Inorganic Pyrophosphatase

Recombinases

Cre Recombinase

ssDNA Binding Proteins

ET SSB

RecA

RecAf

T4 Gene 32 Protein

Kinases

5-hydroxymethyluridine DNA Kinase

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (3' phosphatase minus)

T4 Polynucleotide Kinase Reaction Buffer

DNA Labeling

DNA Polymerase I (E. coli)

Klenow Fragment (3'→5' exo-)

Streptavidin

T4 Polynucleotide Kinase

Terminal Transferase

Therminator™ DNA Polymerase

Other Products

M13KO7 Helper Phage

TelN Protelomerase

β-Agarase I

Argonautes

Tth Argonaute (TtAgo)

DNA Ligases

9°N™ DNA Ligase

Blunt/TA Ligase Master Mix

E. coli DNA Ligase

ElectroLigase®

HiFi Taq DNA Ligase

Hi-T4™ DNA Ligase

Immobilized T4 DNA Ligase

Instant Sticky-end Ligase Master Mix

NEBNext® Quick Ligation Module

NEBridge™ Ligase Master Mix

Quick Blunting™ and Quick Ligation™ Kits

Quick Ligation™ Kit

Salt-T4® DNA Ligase

SplintR® Ligase

StickTogether™ DNA Ligase Buffer

T3 DNA Ligase

T4 DNA Ligase

T4 DNA Ligase Reaction Buffer

T7 DNA Ligase

Taq DNA Ligase

Taq DNA Ligase Reaction Buffer

Thermostable 5´ App DNA/RNA Ligase

DNA Repair Enzymes and Structure-specific Endonucleases

Afu Uracil-DNA Glycosylase (UDG)

Antarctic Thermolabile UDG

APE 1

Endonuclease III (Nth)

Endonuclease IV

Endonuclease V

Endonuclease VIII

EnGen® Mutation Detection Kit

Fpg

hAAG

hSMUG1

Mismatch Endonuclease I

PreCR® Repair Mix

RecA

RecAf

T4 PDG (T4 Endonuclease V)

T7 Endonuclease I

Thermolabile USER® II Enzyme

Thermostable FEN1

Tma Endonuclease III

Topoisomerase I (E. coli)

Tth Endonuclease IV

Uracil Glycosylase Inhibitor (UGI)

Uracil-DNA Glycosylase (UDG)

USER® Enzyme

Methyltransferases

AluI Methyltransferase

BamHI Methyltransferase

CpG Methyltransferase (M.SssI)

dam Methyltransferase

EcoGII Methyltransferase

EcoRI Methyltransferase

GpC Methyltransferase (M.CviPI)

HaeIII Methyltransferase

Hhal Methyltransferase

HpaII Methyltransferase

Human DNA (cytosine-5) Methyltransferase (Dnmt1)

MspI Methyltransferase

TaqI Methyltransferase

Exonucleases and Non-specific Endonucleases

DNase I (RNase-free)

DNase I Reaction Buffer

Exonuclease I (E. coli)

Exonuclease I Reaction Buffer

Exonuclease III (E. coli)

Exonuclease T

Exonuclease V (RecBCD)

Exonuclease VII

Exonuclease VIII, truncated

Lambda Exonuclease

Micrococcal Nuclease

Mismatch Endonuclease I

Mung Bean Nuclease

Nuclease P1

RecJf

T5 Exonuclease

T7 Endonuclease I

T7 Exonuclease

Thermolabile Exonuclease I

Phosphatases & Sulfurylases

Antarctic Phosphatase

Antarctic Phosphatase Reaction Buffer

Apyrase

Pyrophosphatase, Inorganic (E. coli)

Pyrophosphatase, Inorganic (yeast)

Quick CIP

Shrimp Alkaline Phosphatase (rSAP)

Thermostable Inorganic Pyrophosphatase

Recombinases

Cre Recombinase

ssDNA Binding Proteins

ET SSB

RecA

RecAf

T4 Gene 32 Protein

Kinases

5-hydroxymethyluridine DNA Kinase

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (3' phosphatase minus)

T4 Polynucleotide Kinase Reaction Buffer

DNA Labeling

DNA Polymerase I (E. coli)

Klenow Fragment (3'→5' exo-)

Streptavidin

T4 Polynucleotide Kinase

Terminal Transferase

Therminator™ DNA Polymerase

Other Products

M13KO7 Helper Phage

TelN Protelomerase

β-Agarase I