Protocols for Discontinued

• Typical Reaction Conditions for β1-4 Galactosidase (P0730)
• Protocol for use with NEBNext mRNA Library Prep Master Mix Set for Illumina (E6110)
• Please see manual (NEB #E6100) for protocols
• Please see manual (NEB #E6000) for protocols.
• Control Reactions with ProtoScript® M-MuLV Taq RT-PCR Kit
• ShortCut® siRNA Mix Suggested Protocol
• High Efficiency Transformation Protocol (C3016)
• Preparing and Loading Protein Ladders (P7711)
• PCR Amplification (E6120)
• Perform a Second Purification of the Amplified DNA (E6260)
• Protocol for Expression Using T7 Express Iq (C3016)
• Protocol for Expression Using T7 Express Sampler (C3009)
• Protocol for a Typical miRNA Methylation Reaction (M0228)
• mRNA Fragmentation Protocol (E6114)
• mRNA Fragmentation Protocol (E6116)
• miRNA Detection (E3312)
• Ligation of 3´ and 5´ Adaptors (E6120)
• Labeling Proteins in vitro (S9146)
• Labeling Proteins in vitro (S9142)
• Labeling Purified Proteins in vitro (S9235)
• Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9149)
• NEBNext Small Fragment Removal (E6080)
• NEBNext Quick Ligation Module Protocol (E6060)
• NEBNext Small Fragment Removal (E6116)
• Nick Translation and Amplification of Adaptor Ligated DNA (E6260)
• NEBNext Adaptor Ligation (E6116)
• NEBNext End Repair and dA-Tailing (E6116)
• NEBNext Adaptor Ligation (E6080)
• NEBNext End Repair and dA-Tailing (E6080)
• NEBNext End Repair Module Protocol (E6260)
• NEBNext End Repair Module Protocol (E6060)
• Transformation K. lactis YCT284 Competent Cells (C1002)
• Transformation Protocol (C1007)
• Suggested Protocol for Loading a Sample (P7709)
• Suggested Protocol for Loading a Sample (P7702)
• Transfection Guidelines (E3314)
• Western Transfer Protocol for Anti-MBP Antiserum
• TransPass P Protein: Transfection Protocol
• TransPass R2: Plasmid DNA and siRNA Transfection Protocol
• TransPass R2: siRNA Transfection Protocol
• Reaction Protocols for Protein Deglycosylation Mix (P6039)
• Reconstitution of BioLux Cypridina Luciferase Substrate (E3314)
• Protocol I (Luminometers without injectors) (E3308)
• Purification of Adaptor Ligated DNA (E6260)
• Purification of Amplified DNA (E6260)
• Protocol II (Injector-equipped luminometers) (E3308)
• RNase Contamination Assay Kit (E3320)
• Reverse Transcription (E6120)
• Second Strand cDNA Synthesis (E6114)
• Second Strand cDNA Synthesis
• Cellular Labeling (S9218)
• Cellular Labeling (S9142)
• Cellular Labeling (S9216)
• Cellular Labeling (S9139)
• Cellular Labeling (S9126)
• Cellular Labeling (S9137)
• Cellular Labeling (S9131)
• Detection by Fluorescent Imager (P9311)
• Detection using the Phototope-Star Detection Kit
• CLuc Activity Assay Protocol I (Luminometers without injectors) (E3314)
• CLuc Activity Assay Protocol II (Injector-equipped luminometers) (E3314)
• 5 Minute Transformation Protocol (C3009)
• 5 Minute Transformation Protocol (C3016)
• 1: In Vitro Transcription of Long Templates (> 0.3 kb) (E2030)
• 2: In Vitro Transcription of Short Templates (50-300 nt) (E2030)
• Agencourt AMPure XP Bead Clean-up and Size Selection of End Repaired DNA (E6260)
• Adaptor Ligation of End Repaired DNA (E6260)
• Adaptor or Vector Ligation of cDNA Library (E6114)
• Agencourt® Ampure® Bead (Beckman Coulter) Preparation (E6080)
• Agencourt Ampure Beads (Beckman Coulter) Preparation (E6116)
• Labeling Mammalian Cell Lysates (S9146)
• Labeling Mammalian Cell Lysates (S9235)
• Labeling CLIP-tag Fusion Proteins with BC-substrates (S9222)
• Labeling CLIP-tag Purified Protein In Vitro (P9311)
• Instructions for Cellular Labeling (S9215)
• Labeling Proteins in vitro (S9139)
• Labeling proteins in vitro (S9126)
• Labeling Proteins in vitro (S9131)
• Labeling of Proteins in vitro (S9216)
• Labeling of Proteins in vitro (S9218)
• Labeling of Proteins in vitro (S9137)
• High Efficiency Transformation Protocol (C3009)
• First Strand cDNA Synthesis (E6114)
• First Strand cDNA Synthesis Protocols (E6550)
• First Strand cDNA Synthesis
• Library Preparation Using NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1) (E7450)
• Reaction Conditions for Chemical Coupling with CoA-SH (S9352S)
• Labeling of Proteins in vitro (S9215)
• PCR Protocol for Crimson LongAmp™ Taq DNA Polymerase (M0326)
• PCR Amplification with ProtoScript® M-MuLV Taq RT-PCR Kit
• PCR Amplification with AMV LongAmp™ Taq RT-PCR Kit
• Sample Preparation Methods for PicoPLEX WGA Kit
• Pre-Amplification Protocol for PicoPLEX WGA Kit
• Amplification Protocol for PicoPLEX WGA Kit
• First Strand cDNA Synthesis with AMV LongAmp™ Taq RT-PCR Kit
• First Strand cDNA Synthesis with ProtoScript® M-MuLV Taq RT-PCR Kit
• NEBNext End Repair cDNA Library (E6114)
• PCR Amplification of Adaptor Ligated cDNA Library (E6114)
• LongAmp™ Taq 2X Master Mix Protocol (E6060)
• Protocol for Labeling ACP- or MCP-tag Fusion Proteins with CoA Substrates.
• Reaction Conditions for Chemical Coupling (S9237)
• Reaction Conditions for Chemical Coupling (S9155)
• Labeling CLIP-tag Fusion Proteins with BC-substrates (S9237)
• Labeling SNAP-tag Fusion Proteins with BG-substrates (S9155)
• Cell Surface Labeling ACP-Surface Starter Kit
• Labeling of Proteins in vitro for ACP-Surface Starter Kit
• Removal of terminal N-acetylglucosamine from the biantennary N-linked sugars of IgG
• Expression of SNAPf Fusions in SNAPf-H2B (N9186)
• Expression of SNAPf Fusions (N9185)
• Typical Reaction Conditions for α2-3 Neuraminidase (P0728)
• NEBNext® RNase III RNA Fragmentation Module (E6146)
• Reaction Conditions for Chemical Immobilization (S9149)
• Reaction Conditions for Chemical Coupling (S9222)
• Typical Reaction Conditions (P0732)
• Library Preparation Using NEBNext® Small RNA Library Prep Set for SOLiD™ (E6160)
• M13 Titer Protocol
• Double Digest Protocol using One RE-Mix and One Standard Restriction Enzyme
• Loading a Sample (P7708)
• M13 Amplification
• TransPass D2 Protocol 1: Transfection in the presence of serum
• TransPass D2 Protocol 2: Transfection in the absence of serum
• TransPass™ HUVEC Transfection Reagent: Transfection Protocol
• TransPass COS/293: Transfection Protocol
• TransPass D1 Protocol 1: Transfection in the presence of serum
• TransPass D1 Protocol 2: Transfection in the absence of serum
• Cloning of ACP-tag Fusions in pACP-tag(m)-2 (N9322)
• Combination of TransPass D1 or TransPass D2 & TransPass V (M2561)
• Cellular Labeling (S9101)
• Labeling of Proteins in vitro (S9101)
• Expression of ACP-ADRβ2 Fusions (N9321)
• Expression of ACP-tag Fusions (N9322)
• Expression of MCP-GPI (N9320)
• Standard Digest Using RE-Mix®
• Pmal Piii Vector Protocol
• Expression of CLIPf-NK1R (N9216)
• Expression of CLIPf-H2B (N9218)
• Expression of CLIPf-Cox8A (N9217)
• Double Digest Protocol using Two RE-Mix® Enzymes
• Trypsin Digestion Protocol using NEB Trypsin-ultra™ and the FASP Kit
• NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Small Fragment Removal (E6090)
• NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Adaptor Ligation (E6090)
• NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
• PCR Protocol for Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer (M0321)
• PCR Protocol for Crimson Taq DNA Polymerase (M0324)
• PCR Protocol for Crimson Taq DNA Polymerase with (Mg-free) Buffer (M0325)
• Cloning with USER Enzyme
• Cloning of MCP-tag Fusions in pMCP-tag(m) Vector (N9317)
• Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
• Agencourt® Ampure® Beads (Beckman Coulter) Preparation - NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
• Expression of MCP Fusions (N9317)
• View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
• NEBNext DNA Sample Prep Master Mix Set for 454 Fill-in and ssDNA Isolation Module Protocol (E6070, E6071)
• Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
• Protocol for Digestion Prior to droplet digital PCR (ddPCR)