NEB offers a large selection of fluorescent labels (substrates) for CLIP-tag fusion proteins. CLIP-tag™ substrates consist of a fluorophore conjugated to a cytosine leaving group via a benzyl linker. Substrates label the CLIP-tag without the need for additional enzymes. Cell-permeable substrates (CLIP-Cell™) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (CLIP-Surface™) are specific for fusion proteins expressed on the cell surface only.
CLIP-tag™, CLIP-Cell™ and CLIP-Surface™ are trademarks of New England Biolabs, Inc.
- Cellular Labeling (S9217)
- Cellular Labeling (S9219)
- Cellular Labeling (S9232)
- Cellular Labeling (S9233)
- Cellular Labeling (S9234)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9236)
- Labeling of Proteins in vitro (S9217)
- Labeling of Proteins in vitro (S9219)
- Labeling of Proteins in vitro (S9220)
- Labeling of Proteins in vitro (S9221)
- Labeling of Proteins in vitro (S9232)
- Labeling of Proteins in vitro (S9233)
- Labeling of Proteins in vitro (S9234)
- Use with CLIP-tag substrates (S9220)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- Cellular Labeling (S9221)
- Reaction Conditions for Chemical Coupling (S9236)
SNAP-tag® Technologies: Tools to Study Protein Function
Read about the NEB’s set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins.
- Cellular Imaging & Analysis Brochure
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
- Maffei, M., Morelli, C., Graham, E., Patriarca, S., Donzelli, L., Doleschall, B., de Castro, Reis, F., Nocchi, L., Chadick, C.H., Reymond, L., Correa, I.R., Jr., Johnsson, K., Hackett, J.A., Heppenstall, P.A 2019. A ligand based system for receptor specific delivery of proteins Sci Rep. 9(1), PubMedID: 31844114, DOI: 10.1038/s41598-019-55797-1
- Schulz C. and Köhn M. 2008. Simultaneous protein tagging in two colors Chemistry & Biology . 15, PubMedID: 18291310, DOI:
- Gautier A. et al. 2008. An engineered protein tag for multiprotein labeling in living cells Chemistry & Biology . 15, PubMedID: 18291317, DOI:
- Gautier A. et al. 2009. Selective cross-linking of interacting proteins using self-labeling tags J. Am. Chem. Soc. . 131, PubMedID: 19916541, DOI:
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.