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Cellular Analysis

New England Biolabs provides two different protein labeling systems that facilitate the attachment of virtually any molecule to a protein of interest. SNAP- and CLIP-tag protein labeling systems enable the specific, covalent attachment of virtually any molecule to a protein of interest. There are two steps to using this system: cloning and expression of the protein of interest as a SNAP-tag® fusion, and labeling of the fusion with the SNAP-tag substrate of choice. The SNAP-tag is a small protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein. SNAP-tag substrates are dyes, fluorophores, biotin, or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. CLIP-tag™ is a modified version of SNAP-tag, engineered to react with benzylcytosine rather than benzylguanine derivatives. When used in conjunction with SNAP-tag, CLIP-tag enables the orthogonal and complementary labeling of two proteins simultaneously in the same cells. 

Choose Product:

Cellular Analysis

Starter Kits

SNAP-Cell® Starter Kit

SNAP-tag Substrates

BG-GLA-NHS

BG-Maleimide

BG-NH2

BG-PEG-NH2

SNAP-Biotin®

SNAP-Capture Magnetic Beads

SNAP-Capture Pull-Down Resin

SNAP-Cell® 505-Star

SNAP-Cell® Block

SNAP-Cell® Oregon Green®

SNAP-Cell® TMR-Star

SNAP-Cell® 430

SNAP-Cell® 647-SiR

SNAP-Surface® 488

SNAP-Surface® 549

SNAP-Surface® 594

SNAP-Surface® 649

SNAP-Surface® Alexa Fluor® 488

SNAP-Surface® Alexa Fluor® 546

SNAP-Surface® Alexa Fluor® 647

SNAP-Surface® Block

SNAP-Vista® Green

CLIP-tag™ Substrates

BC-NH2

CLIP-Biotin

CLIP-Cell™ 505

CLIP-Cell™ Block

CLIP-Cell™ TMR-Star

CLIP-Surface™ 488

CLIP-Surface™ 547

CLIP-Surface™ 647

SNAP-Capture

SNAP-Capture Magnetic Beads

SNAP-Capture Pull-Down Resin

Blocking Agents

CLIP-Cell™ Block

SNAP-Cell® Block

SNAP-Surface® Block

Cellular Analysis Vectors

pCLIPf Vector

pSNAPf Vector

pSNAP-tag® (T7)-2 Vector

Synthases

Biotin/Vista Labels

CLIP-Biotin

SNAP-Biotin®

SNAP-Vista® Green

Building Blocks

BC-NH2

BG-GLA-NHS

BG-Maleimide

BG-NH2

BG-PEG-NH2

SNAP-tag Other

Anti-SNAP-tag® Antibody (Polyclonal)

SNAP-tag® Purified Protein

Starter Kits

SNAP-Cell® Starter Kit

SNAP-tag Substrates

BG-GLA-NHS

BG-Maleimide

BG-NH2

BG-PEG-NH2

SNAP-Biotin®

SNAP-Capture Magnetic Beads

SNAP-Capture Pull-Down Resin

SNAP-Cell® 505-Star

SNAP-Cell® Block

SNAP-Cell® Oregon Green®

SNAP-Cell® TMR-Star

SNAP-Cell® 430

SNAP-Cell® 647-SiR

SNAP-Surface® 488

SNAP-Surface® 549

SNAP-Surface® 594

SNAP-Surface® 649

SNAP-Surface® Alexa Fluor® 488

SNAP-Surface® Alexa Fluor® 546

SNAP-Surface® Alexa Fluor® 647

SNAP-Surface® Block

SNAP-Vista® Green

CLIP-tag™ Substrates

BC-NH2

CLIP-Biotin

CLIP-Cell™ 505

CLIP-Cell™ Block

CLIP-Cell™ TMR-Star

CLIP-Surface™ 488

CLIP-Surface™ 547

CLIP-Surface™ 647

SNAP-Capture

SNAP-Capture Magnetic Beads

SNAP-Capture Pull-Down Resin

Blocking Agents

CLIP-Cell™ Block

SNAP-Cell® Block

SNAP-Surface® Block

Cellular Analysis Vectors

pCLIPf Vector

pSNAPf Vector

pSNAP-tag® (T7)-2 Vector

Synthases
Biotin/Vista Labels

CLIP-Biotin

SNAP-Biotin®

SNAP-Vista® Green

Building Blocks

BC-NH2

BG-GLA-NHS

BG-Maleimide

BG-NH2

BG-PEG-NH2

SNAP-tag Other

Anti-SNAP-tag® Antibody (Polyclonal)

SNAP-tag® Purified Protein