NEB® 5-alpha Competent E. coli (High Efficiency)

Features

• Versatile cloning strain
• DH5α™ derivative

Application Features

DNA Effects on Transformation Efficiency and Colony Output: 

Effect of heat shock time on NEB 5-alpha competent E.coli transformation efficiency: 

Effect of DNA incubation time on NEB 5-alpha competent E.coli transformation efficiency: 

Effect of outgrowth medium on transformation efficiency: 

Benefit from the high transformation efficiencies of NEB 5-alpha: 

Companion Products

• NEB® 5-alpha Competent E. coli (Subcloning Efficiency)
• NEB® 5-alpha Electrocompetent E. coli
• NEB® 5-alpha F' I^q Competent E. coli (High Efficiency)
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• SOC Outgrowth Medium

1. For C2987H, C2987I, C2987P and C2987U, SOC Outgrowth Medium is provided.
   For C2987R, Outgrowth Medium 1.5 is provided.
   
   
2. CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
   
   
3. STORAGE AND HANDLING:
   Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
   
   SOC medium can be stored at either 4°C or Room Temperature depending on how fast it will be used. Storing at Room Temperature is convenient and adequate for short term usage (weeks to a couple of months). For long term storage, we recommend storing at 4°C. Please note that Outgrowth Medium 1.5 supplied with C2987R (1 x 384 well plate format) must only be stored at Room Temperature or crystals will form.

1. 5 Minute Transformation Protocol (C2987H/C2987I)
2. High Efficiency Transformation Protocol (C2987H/C2987I)
3. High Efficiency Transformation Protocol for 96-well format (C2987P)
4. 14 Minute Transformation Protocol (NEB #C2987H/C2987I)
5. High Efficiency Transformation Protocol for 96-tube format (C2987U)
6. High Efficiency Transformation Protocol for 384-well format (C2987R)

• Additional E. coli Strain Genotypes
• Chemical Transformation Tips
• Convenient Formats of Competent Cells
• Genetic Markers
• McrA, McrBC and EcoKI Strain Phenotypes
• Restriction of Foreign DNA by E. coli K-12

• Enhancing Transformation Efficiency C2987

• Characteristics of Select E.coli Strains
• Cloning Competent Cell Transformation Selection Chart
• Competent Cell Product Comparison
• Competent Cell Selection Guide
• Strain Properties

• Competitor Cross-Reference Tool

• NEBcloner

1. Which competent cell strains are compatible with Gateway® Cloning? Which strains are not compatible?
2. Can I store competent cells at -20°C instead of -80°C?
3. Do you carry competent cells which are compatible with Gateway® Cloning?
4. How can I increase transformation efficiency?
5. How long should I incubate cells on ice after DNA has been added (NEB #C2987H and NEB #C2987I)?
6. How should I calculate the transformation efficiency (C2987)?
7. What are the solutions/recipes (C2987)?
8. What are the strain properties (C2987)?
9. What is the difference between NEB #C2987H and NEB #C2987I?
10. What is the difference between NEB #C2988J and NEB #C2987H?
11. What is the optimal heat shock time for this strain (NEB #C2987H and NEB #C2987I)?
12. What type of competent cells are suitable for transformation of DNA constructs created using Gibson Assembly?
13. Which strain of Competent E. coli should I use for general cloning?
14. Which kind of transformation tubes should be used?
15. What volume of DNA can be added into competent cells?
16. Are NEB's competent cells compatible with the “Mix & Go" protocol?
17. What is the shelf life for this strain (NEB #C2987H and NEB #C2987I)?
18. Can the 96-well plate format of NEB 5-alpha Competent E.coli, NEB #C2987P, be separated into smaller sections?
19. How does the transformation efficiency of the 96-well plate format (NEB #C2987P) compare to the other formats?
20. What is the optimal heat shock time for the 96-well plate format NEB 5-alpha Competent E.coli (NEB #C2987P)?
21. I am competing in the iGEM competition. Do you have any products that I should consider from NEB?
22. What is the optimal heat shock time for the 384-well plate format NEB 5-alpha Competent E. coli (NEB #C2987R)?
23. What is the optimal heat shock time for the 96-tube format NEB 5-alpha Competent E. coli (NEB #C2987U)?
24. What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix?
25. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?

Product Citation Tool

Additional Citations

• Lee S.B., Park S.K., Kim Y.S. (2015) Production of bioactive chicken (Gallus gallus) follistatin-type proteins in E. coli Sci Rep; 5, 11603. PubMedID: 26302688 , DOI: 10.1038/srep11603
• Kostylev M., Otwell A.E., Richardson R., Suzuki Y. (2015) Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies PLoS One; 10, e0137466. PubMedID: PMC4562628 , DOI: 10.1371/journal.pone.0137466
• Mascher G, Derman Y, Kirk DG, Palonen E, Lindström M, Korkeala H (2014) The CLO3403/CLO3404 Two-Component System of Clostridium botulinum E1 Beluga Is Important for Cold Shock Response and Growth at Low Temperatures Appl Environ Microbiol; 80(1), 399-407. PubMedID: 24185852 , DOI: 10.1128/AEM.03204-13

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• C2987H_I_v1
• C2987P_v1
• C2987R_v1
• C2987U_v1
• C2987P_v2
• C2987R_v2
• C2987U_v2
• C2987R_v3

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• C2987H_I_v1_4491604
• C2987H_I_v1_4741606
• C2987H_I_v1_4511606
• C2987H_I_v1_4501607
• C2987H_I_v1_4621607
• C2987H_I_v1_4631607
• C2987H_I_v1_3751506
• C2987H_I_v1_3921508
• C2987H_I_v1_3991509
• C2987H_I_v1_4141510
• C2987H_I_v1_4171511
• C2987H_I_v1_4601607
• C2987U_v1_4641608
• C2987H_I_v1_4661608
• C2987P_v1_4581608
• C2987H_I_v1_4601608
• C2987H_I_v1_4601609
• C2987H_I_v1_4681609
• C2987U_v1_4641609
• C2987H_I_v1_4181512
• C2987H_I_v1_4391603
• C2987H_I_v1_4471604
• C2987H_I_v1_4671609
• C2987H_I_v1_4691609
• C2987H_I_v1_4701609
• C2987H_I_v1_4711609
• C2987H_I_v1_4731609
• C2987P_v1_4581609
• C2987H_I_v1_4741610
• C2987H_I_v1_4791610
• C2987H_I_v1_4821610
• C2987P_v1_4881611
• C2987U_v1_4901611
• C2987H_I_v1_4751610
• C2987H_I_v1_4751611
• C2987H_I_v1_4781612
• C2987H_I_v1_4921611
• C2987H_I_v1_4801611
• C2987H_I_v1_5041612
• C2987U_v1_4901612
• C2987H_I_v1_4951607
• C2987H_I_v1_4591607
• C2987H_I_v1_4831612
• C2987H_I_v1_4851612
• C2987P_v1_4881612
• C2987H_I_v1_4911612
• C2987H_I_v1_4941702
• C2987H_I_v1_4991702
• C2987H_I_v1_5011702
• C2987P_v1_4881703
• C2987H_I_v1_5101701
• C2987H_I_v1_5191702
• C2987U_v1_5121703
• C2987H_I_v1_5021703
• C2987H_I_v1_5051703
• C2987H_I_v1_4951703
• C2987H_I_v1_5091704
• C2987H_I_v1_5131704
• C2987H_I_v1_5201704
• C2987H_I_v1_5071704
• C2987H_I_v1_5151704
• C2987H_I_v1_5341704
• C2987U_v2_5121705
• C2987H_I_v1_5201705
• C2987H_I_v1_5161705
• C2987H_I_v1_5211706
• C2987H_I_v1_5321706
• C2987P_v2_5111706
• C2987U_v2_5201706
• C2987H_I_v1_3331411
• C2987H_I_v1_5221706
• C2987H_I_v1_5261706
• C2987H_I_v1_5311706
• C2987U_v2_5371706
• C2987H_I_v1_5081707
• C2987H_I_v1_5181707
• C2987H_I_v1_5181708
• C2987H_I_v1_5231707
• C2987H_I_v1_5241708
• C2987H_I_v1_5251708
• C2987H_I_v1_5271707
• C2987H_I_v1_5281707
• C2987H_I_v1_5291708
• C2987H_I_v1_5351709
• C2987H_I_v1_5411708
• C2987H_I_v1_5421709
• C2987H_I_v1_5421710
• C2987H_I_v1_5461707
• C2987H_I_v1_5471708
• C2987H_I_v1_5481708
• C2987H_I_v1_5491710
• C2987H_I_v1_5501710
• C2987H_I_v1_5511709
• C2987H_I_v1_5521709
• C2987H_I_v1_5541709
• C2987H_I_v1_5551710
• C2987H_I_v1_5561711
• C2987P_v2_5111708
• C2987P_v2_5111710
• C2987P_v2_5371711
• C2987P_v2_5511711
• C2987U_v2_5371708
• C2987U_v2_5381709
• C2987U_v2_5381710
• C2987U_v2_5501708
• C2987H_I_v1_5571711
• C2987H_I_v1_5611711
• C2987H_I_v1_5621712
• C2987U_v2_5381711
• C2987H_I_v1_5631712
• C2987H_I_v1_5651712
• C2987H_I_v1_5661711
• C2987P_v2_5691711
• C2987U_v2_5631712
• C2987H_I_v1_5721712
• C2987H_I_v1_5351801
• C2987H_I_v1_5431801
• C2987H_I_v1_5771801
• C2987P_v2_5691801
• C2987R_v2_4361801
• C2987U_v2_5771801
• C2987H_I_v1_5711801
• C2987H_I_v1_5661801
• C2987P_v2_5701802
• C2987U_v2_5691802
• C2987H_I_v1_5741802
• C2987H_I_v1_5751802
• C2987H_I_v1_5581801
• C2987H_I_v1_5761802
• C2987H_I_v1_5861802
• C2987H_I_v1_5821802
• C2987H_I_v1_5781803
• C2987U_v2_5871803
• C2987H_I_v1_5731802
• C2987H_I_v1_5791803
• C2987H_I_v1_5891803
• C2987H_I_v1_5891804
• C2987P_v2_5701803
• C2987H_I_v1_5801803
• C2987H_I_v1_5841803
• C2987H_I_v1_5851803
• C2987H_I_v1_6011804
• C2987P_v2_5961803
• C2987H_I_v1_5901804
• C2987P_v2_5961805
• C2987H_v1_10010333
• C2987I_v1_10010383
• C2987P_v2_10009786
• C2987R_v3_10011055
• C2987R_v3_10011065
• C2987U_v2_10010008
• C2987U_v2_10011740
• C2987H_v1_10009483
• C2987H_v1_10011531
• C2987I_v1_10012788
• C2987P_v2_10012897
• C2987U_v2_10013428
• C2987H_v1_10013422
• C2987I_v1_10013998
• C2987P_v2_10014299
• C2987U_v2_10014835
• C2987H_v1_10014830
• C2987H_v1_10015491
• C2987I_v1_10015844
• C2987U_v2_10016263
• C2987H_v1_10017212
• C2987I_v1_10017213
• C2987H_v1_10016602
• C2987U_v2_10018953
• C2987H_v1_10018134
• C2987I_v1_10019407
• C2987P_v2_10018930
• C2987P_v2_10019403
• C2987H_v1_10019405
• C2987U_v2_10019408
• C2987H_v1_10020248
• C2987I_v1_10020865
• C2987H_v1_10021340
• C2987P_v2_10022380
• C2987H_v1_10022954
• C2987I_v1_10022962
• C2987U_v2_10022964
• C2987H_v1_10023817
• C2987P_v2_10023819
• C2987U_v2_10024314
• C2987I_v1_10024960
• C2987P_v2_10025678
• C2987H_v1_10025350
• C2987H_v1_10025668
• C2987I_v1_10026485
• C2987H_v1_10027607
• C2987U_v2_10028053
• C2987H_v1_10028621
• C2987P_v2_10029519
• C2987U_v2_10029520
• C2987H_v1_10029525
• C2987I_v1_10029660
• C2987U_v2_10030700
• C2987H_v1_10030906
• C2987H_v1_10032170
• C2987I_v1_10031575
• C2987P_v2_10032354
• C2987H_v1_10032812
• C2987H_v1_10033400
• C2987I_v1_10033101
• C2987P_v2_10033922
• C2987U_v2_10032816
• C2987H_v1_10034313
• C2987I_v1_10034342
• C2987U_v2_10035218
• C2987H_v1_10035214
• C2987H_v1_10035965
• C2987H_v1_10036613
• C2987I_v1_10035971
• C2987P_v2_10036215
• C2987U_v2_10036762
• C2987H_v1_10037275
• C2987I_v1_10037276
• C2987H_v1_10037877
• C2987U_v2_10038803
• C2987H_v0_10041467
• C2987H_v1_10039040
• C2987H_v1_10039967
• C2987I_v1_10039409
• C2987P_v2_10037875
• C2987P_v2_10041108
• C2987U_v2_10040975
• C2987H_v0_10041894
• C2987I_v1_10041468
• C2987U_v2_10042152
• C2987H_v1_10042398
• C2987I_v1_10042399
• C2987P_v2_10043194
• C2987H_v1_10043193
• C2987H_v1_10044331
• C2987I_v1_10044490
• C2987U_v2_10044332
• C2987H_v1_10044808
• C2987P_v2_10044805
• C2987U_v2_10044809
• C2987H_v1_10045594
• C2987H_v1_10046067
• C2987I_v1_10045308
• C2987P_v2_10045595
• C2987H_v1_10046411
• C2987H_v1_10047057
• C2987I_v1_10046413
• C2987P_v2_10046730
• C2987P_v2_10047647
• C2987U_v2_10046414
• C2987R_v3_10048062
• C2987H_v1_10048005
• C2987I_v1_10047646
• C2987H_v1_10048233
• C2987P_v2_10048961
• C2987U_v2_10048962
• C2987I_v1_10049543
• C2987H_v1_10049345
• C2987I_v1_10050822
• C2987H_v1_10050479
• C2987H_v1_10051920
• C2987U_v2_10051985
• C2987H_v1_10052123
• C2987H_v1_10052230
• C2987I_v1_10052220
• C2987P_v2_10051984
• C2987U_v2_10053490

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEB® 5-alpha Competent E. coli (High Efficiency)
• SOC Outgrowth Medium
• pUC19 Vector

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.