K. lactis Protease Deficient Competent Cell Sampler
- This product was discontinued on September 15, 2014
Chemically competent Kluyveromyces lactis cells are suitable hosts for expressing proteins that have exhibited proteolysis in the parental GG799 background (NEB #C1001S). Proteolysis of secreted heterologous proteins in K. lactis is most often casued by aspartyl poroteases that are present in the secretory pathway. In many cases, a single aspartyl protease causes the majority of the detrimental protein degredation. The Protease Deficient Competent Cell Sampler contains a wild-type GG799 strain, as well as four protease deficient K. lactis strains, each having selectable marker-free deletion of a specific secretory pathway aspartyl protease gene. Each strain can be transformed with any linearized pKLAC-series expression vector, permitting a comparison of heterologous proein quality and yield in each background.
The Sampler contains one transformation each of five K.lactis strains. K. lactis GG799 is a wild-type expression strain. K. lactis YCT389 contains a selectable marker-free deletion of the KLLA0E0398g locus encoding an extracellular aspartyl protease (KlYps1p) that is homologous to cerevisiae Yps1p. K. lactis YCT390 carries a selectable marker-free deletion of the KLLA0F2208g locus encoding an extracellular aspartyl protease (Klyps7p) that is homologous to S.cervisaiae Yps7p. K. lactis strain YCT 569 carries a selectable marker-free deletion of the KLLA0D01507g locus encoding an extracellular aspartyl protease belonging to pfam00026. K. lactis strain YCT598 carries a selectable marker-free deletion of the KLLA0D15917g locus encoding an extracellular aspartyl protease that is homologous to S.cervisaiae Bar1p.
The following reagents are supplied with this product:
Store at (°C) Concentration NEB® Yeast Transformation Reagent (5 ml) 4 1 X
- Product Categories:
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.