ThermoPol^® Reaction Buffer

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
B9004S     -20       ThermoPol® Reaction Buffer B9004SVIAL -20 4 x 1.5 ml 10 X   Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM

Materials Sold Separately

• Magnesium Sulfate (MgSO₄) Solution

• Activity of Restriction Enzymes in PCR Buffers

• NEB Diluent and Buffer Table

• PCR Selector
• Tm Calculator
• Tm Calculator

1. What is supplied with the ThermoPol Reaction Buffer pack?
2. What is the composition of the ThermoPol buffer?
3. Which Polymerases use this reaction buffer?
4. How should the NEBuffer be used?
5. The buffer arrived thawed.  Are the buffer and the enzyme still active?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• B9004S_v1
• B9004S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• B9004S_v1_0021701
• B9004S_v1_0031712
• B9004S_v1_10013477
• B9004S_v1_10019255
• B9004S_v1_10026022
• B9004S_v1_10029070
• B9004S_v1_10031992
• B9004S_v1_10034370
• B9004S_v1_10035285
• B9004S_v1_10042722
• B9004S_v1_10044651
• B9004S_v1_10048538
• B9004S_v1_10053461
• B9004S_v1_10056502
• B9004S_v1_10060083
• B9004S_v1_10062930
• B9004S_v1_10066280
• B9004S_v2_10065438
• B9004S_v2_10076518
• B9004S_v2_10086109
• B9004S_v2_10092719
• B9004S_v2_10098075
• B9004S_v2_10103186
• B9004S_v2_10109578
• B9004S_v2_10121008
• B9004S_v2_10122155
• B9004S_v2_10132252
• B9004S_v2_10118448
• B9004S_v2_10139955
• B9004S_v2_10145255
• B9004S_v2_10145926
• B9004S_v2_10150531
• B9004S_v2_10158954
• B9004S_v2_10163703
• B9004S_v2_10167673
• B9004S_v2_10176826
• B9004S_v2_10183495

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• ThermoPol^® Reaction Buffer
• Magnesium Sulfate (MgSO₄) Solution

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

NEW ENGLAND BIOLABS®, VENT® and THERMOPOL® are registered trademarks of New England Biolabs, Inc. 
DEEP VENT® is a trademark of New England Biolabs, Inc. 

TRITON® is a registered trademark of Union Carbide Corporation.