Red Loading Buffer Pack

An alternative product is Blue Loading Buffer (NEB #B7703)

  • Catalog # B7709 was discontinued on November 13, 2017

The red loading buffer pack contains one vial of red loading buffer and one vial of 30X reducing agent. The combined solution is ideal for protein gel applications.

  • 1X Red Loading Buffer Composition: 187.5 mM Tris-HCl (pH 7.0), 6% SDS (w/v), 30% glycerol, 0.03% phenol red (w/v)
  • 30X Reducing Agent: 1.25 M DTT
  • SDS interacts with positively charged amino acids of proteins, thereby disrupting interactions that form protein structures to separate proteins based on size rather than charge or shape 
  • DTT is present to help reduce structural disulfide bonds
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