Monarch^® gDNA Blood Lysis Buffer

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
T3013L     25       Monarch® gDNA Blood Lysis Buffer T3013-2 25 1 x 20 ml Not Applicable

1. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
2. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
3. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
4. Genomic DNA Purification from Yeast (NEB #T3010)
5. Genomic DNA Purification from Saliva (NEB #T3010)
6. Protocol for Genomic DNA Cleanup (NEB #T3010)
7. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)
8. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
9. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
10. Quick Protocol for Extraction and Purification of Genomic DNA

• Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
• Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
• Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

1. What is the composition of each buffer provided with the Monarch^® Genomic DNA Purification Kit?
2. What size gDNA can be purified with the Monarch^® Genomic DNA Purification Kit?
   
    
3. Can I omit the RNase digestion step from the protocol? 
4. Does it matter which anticoagulant is used in blood samples?

• Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)

• Monarch Nucleic Acid Purification Brochure

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T3013L_v1
• T3013S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T3013L_v1_10028543
• T3013L_v1_10041705
• T3013L_v1_10068614
• T3013L_v1_10106823
• T3013L_v1_10119658
• T3013L_v1_10144596
• T3013L_v2_10144617
• T3013L_v2_10163991
• T3013L_v2_10204306

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Monarch^® gDNA Blood Lysis Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.