NEBuilder® HiFi DNA Assembly
Choose NEBuilder HiFi
NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible mix enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.
Enjoy simple and fast seamless cloning in as little as 15 minutes
NEBuilder® Assembly Tool
NEBuilder Assembly Tool can be used to design primers for your NEBuilder HiFi DNA Assembly or Gibson Assembly® reactions.
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Bridging dsDNA with a ssDNA oligo and NEBuilder HiFi to create an sgRNA-Cas9 Expression Vector
NEB has developed a protocol for construction of an sgRNA or sgRNA library into a Cas9/sgRNA expression vector using single-stranded DNA oligonucleotides, a restriction enzyme digested vector and the NEBuilder HiFi DNA Assembly Master Mix.
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Improved methods for site-directed mutagenesis using NEBuilder HiFi DNA Assembly Master Mix
Here we describe the use of the NEBuilder HiFi DNA Assembly Master Mix to generate multiple site-directed mutagenesis at the same time.
View Application NoteFeatured NEBuilder Products
NEBuilder HiFi DNA Assembly mix is available alone or bundled with competent cells. Please inquire for large volume purchases.
NEBuilder HiFi DNA Assembly Cloning Kit
NEBuilder HiFi DNA Assembly Cloning Kit
This kit includes NEBuilder HiFi DNA Assembly Master Mix and NEB® 5-alpha Competent E. coli (NEB #C2987) for seamless assembly of multiple DNA fragments.
NEBuilder HiFi DNA Assembly Bundle for Large Fragments
NEBuilder HiFi DNA Assembly Bundle for Large Fragments
This bundle for DNA assembly of large fragments includes NEB 10-beta Competent E. coli, High Efficiency (NEB #C3019) with the NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) for assemblies larger than 15 kb.
NEBuilder HiFi DNA Assembly Master Mix
NEBuilder HiFi DNA Assembly Master Mix
This Master Mix allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility.
This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
