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NEBNext Ultra II Directional RNA libraries have high directionality/strandedness
Dir_RNA_Libraries_Figure2
Ribosomal RNA (rRNA) was depleted from Human Universal Reference RNA (Agilent #740000) and libraries were made using 1 µg, 100 ng and 5 ng input with NEBNext Ultra II Directional RNA Kit (plus the NEBNext rRNA Depletion Kit (Human/Mouse/Rat)), and using 1 µg and 100 ng input with Kapa Stranded RNA-Seq Kit with RiboErase, Kapa HyperPrep Kit with RiboErase, and Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero™ Gold. Libraries were sequenced on an Illumina NextSeq® 500 using paired-end mode (2x76 bp). Reads were mapped to the hg19 reference genome using Hisat 2.0.3 and directionality was calculated using the infer experiment tool from the RSeQC Quality Control Package. Ultra II Directional RNA retains high directionality at low ng input amounts.

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Available Kits:

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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.
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NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.