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The Monarch® HMW DNA Extraction Kits provide a rapid and reliable process for extracting high molecular weight DNA (HMW DNA) from biological samples. These kits utilize an optimized process that combines gentle cell lysis with a tunable fragment length generation, followed by precipitation of the extracted DNA onto the surface of large glass beads. DNA size ranges from 50-250 kb for the standard protocol and into the Mb range for certain sample types when the lowest agitation speeds are used. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA, and is ready for use in downstream applications including long-read sequencing.
Reasons to Choose the Monarch HMW DNA Extraction Kits
- Extremely fast, user-friendly protocols utilizing a novel glass-bead-based approach
- Reproducibly purify high molecular weight genomic DNA (HMW DNA) from various sample types
- Tune fragment length by varying agitation speeds during lysis; achieve DNA in the Megabase size range with low speeds
- Achieve outstanding results when compared to other commercially available solutions
- Excellent performance in long read sequencing
Extremely fast, user-friendly protocols utilizing a novel glass-bead-based approachComparison of HMW DNA Extraction Methods
|Extraction Method||Approximate protocol/workflow time*||Notes**|
|NEB Monarch HMW DNA Extraction||30 minutes||60 minutes||90 minutes||Fast, convenient, High yield, pure, tunable DNA size|
|Circulomics® Nanobind®||> 60 minutes||60 minutes||>2 hours||Several hands-on steps, difficult to process some samples due to high viscosity.|
|Phenol/Chloroform Extraction||> 6 hours||> 6 hours||> 6 hours||Hazardous reagents, lengthy workflow, DNA is difficult to dissolve|
|Qiagen® MagAttract®||Not compatible||~60 minutes||Overnight
+ 40 minutes
|DNA is heavily sheared|
|Qiagen® Genomic Tips||~3.5 hours||3.5 hours||~4 hours||Long workflow, sub-optimal yields, not very high molecular weight|
|Revolugen® FireMonkey®||~45 minutes||>60 minutes||Not compatible||Only second eluate is used (very low recovery); DNA heated to 80°C leading to denaturing|
**Notes are based on internal testing or usage
Tune fragment length by varying agitation speeds during lysis; achieve DNA in the Megabase size range with low speeds
Achieve outstanding results when compared to other commercially available solutions
Excellent performance in long read sequencing
|HEK293 SAMPLE 1||HEK293 SAMPLE 2||HUMAN BLOOD SAMPLE 1||HUMAN BLOOD SAMPLE 2||MOUSE KIDNEY SAMPLE|
|Mean read length (bases)||21338.9||19249.9||21522.6||24677.7||27120.7|
|Mean read quality||12.8||13.2||13.4||13.3||13|
|Median read length||10388||9702||10130||12593||23150|
|Median read quality||13.2||13.7||13.9||13.8||13.5|
|Number of reads||377687||633636||538090||327314||164000|
|Read length N50 (bases)||45432||40415||46542||51394||44631|
Overview of High Molecular Weight DNA Extraction using the Monarch HMW DNA Extraction Kit for Cells & Blood
Visualize how HMW DNA is extracted from cells and blood with the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050).
In this webinar, the developer of Monarch HMW DNA Extraction Kits introduces the technology, the workflow and subsequent long read sequencing data.
In this video, we walk through the protocol for extraction of high molecular weight DNA (HMW DNA) from cultured cells.
In this video, we walk through the protocol for extraction of high molecular weight DNA (HMW DNA) from tissues.
In this video, we share some important tips for successfully extracting high molecular weight DNA (HMW) from blood samples.
We’ve had great success with obtaining HMW DNA for long-read sequencing from a variety of cell types, using less input and obtaining a comparable yield…It is straightforward and easy to use.
Other Monarch Kits Available:
Featured Tools and Resources
|A faster workflow for the assessment of genomic loci in mice using a novel HMW DNA extraction technology upstream of Cas9 targeted sequencing|
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