While cloning has typically been a time-consuming process in the past, advances in both technologies and reagents have enabled the process to take much less time. Race to NEB for the highest quality products that save valuable time!

 

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Fast cloning reagents from NEB:

 

SEAMLESS CLONING

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NEBuilder® HiFi DNA Assembly Master Mix, NEBuilder HiFi DNA Assembly Cloning Kit & NEBuilder HiFi DNA Assembly Bundle for Large Fragments

Rapid, seamless assembly/cloning of multiple fragments in as little as 15 minutes

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NEB® Gibson Assembly Master Mix &
Gibson Assembly Cloning Kit

Successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility

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NEB® Golden Gate Assembly Mix

Efficient, ordered assembly of multiple fragments in a single reaction

DIGESTION

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High-Fidelity (HF®) restriction enzymes

HF enzymes offer reduced star activity, rapid digestion and 100% activity in CutSmart® Buffer 

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Time-Saver™ restriction enzymes

>195 enzymes are Time-Saver qualified and will digest DNA in 5-15 minutes; digest overnight with no unwanted star activity

END MODIFICATION

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Quick Dephosphorylation Kit

Complete dephosphorylation in 10 minutes with rapid, irreversible heat inactivation

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Quick Blunting Kit

Blunt restriction enzyme-digested DNA in less than 30 minutes at room temperature

AMPLIFICATION

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Q5® High-Fidelity DNA Polymerases

With the lowest error rate of all known commercially available PCR polymerases, Q5 enables extension times as low as 10 seconds per kb on templates up to 20 kb

PURIFICATION

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Monarch® DNA Purification kits

Purify high-quality DNA in minutes (5 minutes for the PCR & DNA Cleanup Kit!). Designed with sustainability in mind, Monarch kits use less plastic and responsibly-sourced packaging

LIGATION

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Instant Sticky-end Ligase Master Mix

Specially formulated to instantly ligate 2-4 basepair overhangs! Just add DNA, flick the tube, and ligation occurs instantly! No need for incubation.

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Blunt/TA Ligase Master Mix

Specially formulated to ligate single-base and blunt ends in just 15 minutes

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Quick Ligation Kit

Ligates both cohesive and blunt ends in just 5 minutes at room temperature

TRANSFORMATION

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NEB Turbo Competent E. coli (High-Efficiency)

Generate colonies in as little as 6.5 hours with a 5-minute transformation protocol

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One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications

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Featured fast cloning resources:

ONLINE TOOLS

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NEBcloner®

For products, protocols and tips for each step of your traditional cloning experiment

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NEBioCalculator®

For help with scientific calculations and conversions for DNA and RNA

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NEBcutter®

To identify the restriction sites within your DNA sequence

BROCHURES & TECHNICAL GUIDES

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Molecular Cloning Technical Guide

Get help with product selection, protocols, tips for optimization and troubleshooting.

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Reagents & Tools for Molecular Cloning Brochure

Learn about NEB's recommended products for cloning.