FAQ: What is the difference between EnGen® Spy Cas9 HF1 and EnGen Spy Cas9 NLS (NEB #M0646)?

EnGen Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage.