HomeFAQsShould I include a No-RT control reaction?
FAQ: Should I include a No-RT control reaction?
Yes. The existence of residual amount of genomic DNA or carry-over products can interfere with downstream detection. Therefore, it is important to carry out the appropriate No-RT control reactions to account for these effects. Treating your RNA samples with DNaseI (NEB #M0303) will help remove contaminant DNA prior to performing cDNA synthesis.