Yes. Most PCR buffers are compatible. No additional reaction buffer is necessary if the enzyme is added to a PCR reaction.
Incubation in the following buffers at 55°C for 15 minutes resulted in the relative activity listed:
| NEBuffer | % Activity |
|---|---|
| r1.1 (NEB# B7030) | 20 |
| r2.1 (NEB# B6002) | 50 |
| r3.1 (NEB# B6003) | 50 |
| rCutSmartTM (NEB# B6004) | 100 |
| Exonuclease I Reaction Buffer (NEB# B0293) | 10 |
| ThermoPol (NEB# B9004) | 90 |
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