HomeFAQsCan Faustovirus Capping Enzyme (NEB #M2081) use cap analogs in a capping reaction?
FAQ: Can Faustovirus Capping Enzyme (NEB #M2081) use cap analogs in a capping reaction?
No. Faustovirus Capping Enzyme uses GTP and SAM to generate a cap structure on 5′ triphosphate RNA, typically a synthetic transcript generated by in vitro transcription. Cap analogs such as GpppG (NEB #S1407), m7GpppG (NEB #S1404), m7GpppA (NEB #S1405S) and anti-reverse cap analog (ARCA; NEB #S1411), are directly incorporated into the transcript by an RNA polymerase as the first nucleotide at the 5′ end.