HomeFAQsI am having trouble amplifying a product that is longer than 1 kb. How can I optimize my product yield using Q5® Blood Direct 2X Master Mix?
FAQ: I am having trouble amplifying a product that is longer than 1 kb. How can I optimize my product yield using Q5® Blood Direct 2X Master Mix?
Try increasing or decreasing input material (whole blood cells). Increase the number of cycles to 40. Lengthen extension time to 30-90 sec/kb.
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