FAQ: Do Dried Blood Spot punches need to be pre-treated before PCR with Q5® Blood Direct 2X Master Mix?

Pre-treatment is generally not necessary for a single 0.5 mm punch in a 20 µL reaction or a single 1 mm punch in a 50 µL reaction. However, when using more or larger punches, they can be pre-treated to avoid possible inhibition of PCR.