FAQ:  What advantages does WarmStart® Nt.BstNBI provide?

WarmStart® Nt.BstNBI can be used in applications that Nt.BstNBI is used. The aptamer in WarmStart Nt.BstNBI inhibits nicking activity below 40°C, therefore the enzyme only becomes fully activated when reaction temperatures of 50-60°C are reached. This feature allows high-throughput assay assembly and room temperature setup (such as SDA). In addition, since the enzyme activity is precisely controlled, the results are consistent and variations between replicates are low.