HomeFAQsMy lysate is viscous; how can I resolve this?
FAQ: My lysate is viscous; how can I resolve this?
A viscous lysate results from an abundance of DNA released by the Lysis Reagent. Shear the DNA by passing the lysate through a 20-gauge needle via syringe several times. Alternatively, adding DNase I (NEB# M0303) at 10 to 100 U/mL, after addition of the Lysis Reagent will reduce the viscosity. It’s recommended to perform a small-scale lysis test with the Lysis Reagent to determine whether DNase I is necessary followed by scale up. NEB’s DNase I is fused to maltose-binding protein (MBP), making its removal via amylose resin (NEB# E8021), amylose resin high flow (NEB# E8022), or amylose magnetic beads (NEB# E8035) simple and effective.