FAQ: Can I add NEBExpress® E. coli Lysis Reagent directly to my cell culture to lyse the cells?

Yes, we recommend using a 1:1 ratio of Lysis Reagent to cell culture or to washed and concentrated cells in a desired buffer as long as the buffer is compatible with downstream steps. NEBExpress® T4 Lysozyme (NEB #P8115) and Micrococcal Nuclease (NEB #M0247S), may also be added if needed.