Home FAQs For what type of sample prep/library construction can I use the NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina?

FAQ: For what type of sample prep/library construction can I use the NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina?

The NEBNext Ultra II DNA PCR-free Library Prep kit for Illumina is compatible with protocols requiring a 3´ A overhang on the library molecules to facilitate ligation to adapters containing a 3´ T overhang, such as library prep for sequencing on the Illumina platform. At this time we have validated the Ultra II DNA PCR-free Library Prep Kit only on the Illumina platform.