Home FAQs What is the lowest RNA input amount recommended for the LunaScript Multiplex One-Step RT-PCR Kit?

FAQ: What is the lowest RNA input amount recommended for the LunaScript Multiplex One-Step RT-PCR Kit?

Successful target amplification depends on the quality of RNA samples and the abundance of the target gene expression. For example, a 570 bp GAPDH amplicon was detected from as low as 0.01 pg of high-quality human total RNA. Successful NGS libraries were also generated from as few as 10 copies of SARS-CoV-2 synthetic RNA using the ARTIC protocol.