RNA from a variety of sources can be used with the NEBNext Immune Sequencing Kit (Human), including:
- High quality RNA from B or T cells. The RNA should have a RIN score ≥7 to provide the highest percentage of full-length transcripts for library construction. When RNA is used with RIN scores <4, the complexity of the library is greatly reduced.
- RNA from any human sample that contains either B or T cells, such as peripheral blood PBMC or tissue.
- RNA extracted from frozen cell pellets, cultured cells, or cells sorted for specific B- or T-cell types.