Home FAQs If I am using 96 samples, can I skip the cleanup step after the Targeted cDNA Amplification?

FAQ: If I am using 96 samples, can I skip the cleanup step after the Targeted cDNA Amplification?

For large numbers of samples, the cleanup step after Targeted cDNA Amplification (section 2.5 in the manual) can be skipped and cDNA samples can be used directly for NEBNext End Prep (section 4.1). This is consistent with the ARTIC Network nCoV-2019 sequencing protocol v3 (LoCost) protocol.