Home FAQs Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Sets 1-4) on the Illumina platforms? 

FAQ: Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Sets 1-4) on the Illumina platforms? 

No. Libraries made NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptor DNA Sets 1-4) are fully compatible with Illumina sequencing protocols of TruSeq HT libraries. For HiSeq, HiScanSQ, or GAIIx systems on single-read flow cells, the TruSeq Dual Index Sequencing Primer Box, Single Read (single use box) (catalog # FC-121-1003) will need to be ordered separately when using both indices. This add-on box is not required with the MiSeq System or on paired-end flow cells for HiSeq, HiScanSQ, GAIIx.  Please refer to Illumina website for complete information.