HomeFAQsHow much sample can be added to the colorimetric LAMP reaction as input?
FAQ: How much sample can be added to the colorimetric LAMP reaction as input?
For best results, we recommend 2 µl of extracted nucleic acid in a 25 µl reaction. Larger or smaller volumes of samples may be tolerated. Ensure the nucleic acid is eluted or prepared in water to avoid carrying over excess buffer to the reaction. Material eluted in TE or similar elution buffer should be kept to less than 5 µl (20% v/v) of the final reaction volume. Samples in transport media (VTM or UTM) should be kept to less than 2 µl (8% v/v). To increase sample volume, use 0.1X Elution Buffer or water for preparation of the nucleic acid.