HomeFAQsAfter amplification my samples turned orange rather than yellow. Is this acceptable? How do I interpret the results?
FAQ: After amplification my samples turned orange rather than yellow. Is this acceptable? How do I interpret the results?
An orange color following incubation at 65°C for 30 minutes suggests amplification occurred but the pH did not decrease sufficiently to turn the reaction yellow. This may be due to inhibition from the input sample, buffering from the input sample or indicates the sample contains a low amount of target nucleic acid. It may be interpreted as positive if all samples were pink prior to amplification and post amplification, both the IC and PC reactions are yellow/orange and the NTC is pink.