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FAQ: What methods can be used to purify the synthesized protein?

Any affinity purification method can be applied, as none of the endogenous proteins in the NEBExpress Cell-free E. coli Protein Synthesis System are tagged. If the synthesized protein contains a His-tag, NEBExpress Ni-NTA Magnetic Beads (NEB #S1423), NEBExpress Ni Spin Columns (NEB #S1427) or NEBExpress Ni Resin (NEB #S1428) can be used.  If the synthesized protein contains a maltose-binding protein (MBP) tag, Amylose Resin (NEB #E8021), Amylose Resin High Flow (NEB #E8022) or Amylose Magnetic Beads (NEB #E8035) can be used. If the synthesized protein has a chitin binding domain (CBD) tag, Chitin resin (NEB #S6651) or Chitin Magnetic Beads (NEB #E8036) can be used.