We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. To learn more and manage cookies, please refer to our Cookie Statement.
HomeFAQsHow many cells should I use for reliable detection?
FAQ: How many cells should I use for reliable detection?
The Luna Cell Ready Lysis Module has a lysis capacity of 10 - 100,000 cells in a 50 µl lysis reaction. Typically, 1-2 µl cell lysate (equivalent to RNA from 0.2 - 4,000 cells) can be transferred into a 20 µl downstream RT-qPCR). Most transcripts can be detected from 20 to 200 cells in a typical 20 µl reaction. However, some cell lines may contain high levels of inhibitors that affect cell lysis or the RT-qPCR reaction. For a quick check of gene expression, ~1000 cells per 50 μl reaction is a good starting point for optimization. Please see additional FAQ’s for guidance regarding specific cell lines.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
