HomeFAQsWhat is the difference between the unit definitions of T4 DNA Ligase (NEB #M0202) and Salt-T4 DNA Ligase (NEB #M0467)?
FAQ: What is the difference between the unit definitions of T4 DNA Ligase (NEB #M0202) and Salt-T4 DNA Ligase (NEB #M0467)?
Both T4 DNA Ligase and Salt-T4 DNA Ligase unit definitions are defined as 50% ligation of 0.12 μM HindIII fragments, however, the incubation time/temperature, buffer conditions as well as the method of detection of ligation products differs. The T4 DNA Ligase unit definition is determined after 30 minutes at 16°C, while the Salt-T4 DNA Ligase unit is determined after 10 minutes at 25°C. The T4 DNA Salt-T4 DNA Ligase and T4 DNA Ligase have the same specific activity (1 unit of Salt-T4 DNA Ligase in 1X T4 DNA Ligase Buffer + 100 mM NaCl=1 unit of T4 DNA Ligase in 1X T4 DNA Ligase Buffer).
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.