HomeFAQsWill extended binding increase the yield of the target protein in the eluate?
FAQ: Will extended binding increase the yield of the target protein in the eluate?
Binding of some His-tagged proteins can be increased with longer incubation times. The column should be placed at 4°C when extending the binding time to maintain activity of the target protein. Prolonged incubation may result in more non-specific binding.
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