If planning to use the higher concentration enzyme for in vitro digestion of DNA, the enzyme can be diluted to 1 μM in 1X Buffer r3.1 and used immediately. The 1 μM dilution in 1X NEBuffer r3.1 should not be frozen.
If the 1 μM dilution will be stored at -20°C, it should be diluted using Diluent B (NEB #B8002S): 300 mM NaCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 500 μg/ml BSA and 50% glycerol (pH 7.4 @ 25°C) prior to the reaction assembly.
