Home FAQs What is known about binding in the presence of nonionic detergents?

FAQ: What is known about binding in the presence of nonionic detergents?

Some fusion proteins do not bind efficiently (< 5% binding) in the presence of 0.2% Triton X–100 or 0.25% Tween 20, while other fusions are unaffected. For one fusion that does not bind in 0.25% Tween 20, diluting the Tween to 0.05% restores about 80% of the binding.