FAQ: Can I substitute a different buffer and/or salt concentration in the Column Buffer?

Yes, we have tried HEPES, MOPS, and phosphate buffers (at pH's from 7.0 to 8.5) instead of Tris-HCl in the Column Buffer with similar results. NaCl or KCl concentrations of 25 mM to 1 M are also compatible with the affinity purification.