FAQ: Can I use a different elution buffer than the one supplied with the Monarch Genomic DNA Purification Kit (NEB #T3010)?

The elution buffer supplied with the kit is 10 mM Tris-HCl, pH 9.0, 0.1 mM EDTA. This buffer is optimal for long term storage of gDNA. Any low-salt buffer can be used for elution, such as 10 mM Tris-HCl pH 8.5, TE Buffer or nuclease-free water. Preheating the elution buffer to 60°C ensures rapid and efficient elution of the gDNA. 

For more information, see “Considerations for Elution and Storage” in the product manual.