HomeFAQsHow important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
FAQ: How important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
The pre-heating of the elution buffer is essential for maximal recovery with the Monarch® Genomic DNA Purification Kit. If more convenient, a temperature of 56°C can be used but may result in a very slight reduction in yield. Room temperature elution buffer can also be used but will result in a 20-30% reduction in yield and may require a second elution. Additionally, at room temperature, large gDNA fragments will elute less efficiently, so the eluted gDNA will have a smaller average size. For more information, see “Considerations for Elution and Storage” in the product manual.