Thermolabile USER II Enzyme is 100% active in a variety of buffers, including CutSmart Buffer (NEB #B7204), NEBuffer 1.1 (NEB #B7201), T4 DNA Ligase Reaction Buffer (NEB #B0202) and TE Buffer (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA). Activity in NEBuffers is as follows:
Additionally, Thermolabile USER II Enzyme is 100% active in many polymerase and PCR buffers (see chart below).
|Buffer||Activity (Relative to T4 DNA Ligase Buffer)|
|Thermopol Reaction Buffer (NEB #B9004)||100%|
|Q5® Reaction Buffer (NEB #B9027)||100%|
|Phusion® HF Buffer (NEB #B0518)||100%|
|PCR Buffer (Roche)||100%|
|GoTaq® Buffer (Promega®)||100%|
|PCR Buffer (Qiagen®)||100%|
|Pfu Turbo Cx Buffer (Agilent Technologies)||100%|
|AmpliTaq Gold® 360 Buffer (ThermoFisher®)||100%|
|Fast Start™ Taq DNA Pol PCR Buffer (Sigma Aldrich)||100%|
|PCR Buffer (Applied Biosystems)||100%|
|PCR Buffer (Enzymatics)||100%|
AmpliTaq Gold®, Phusion® and Thermo Fisher® are registered trademarks and property of Thermo Fisher Scientific.
s.GoTaq® and Promega® are registered trademarks of Promega, Inc.
Qiagen® is a registered trademark of Qiagen, Inc.
Fast Start™ is a trademark of Sigma Aldrich.