HomeFAQsAt what temperature is Hi-T7 RNA Polymerase active?
FAQ: At what temperature is Hi-T7 RNA Polymerase active?
Hi-T7 RNA Polymerase is active between 37°C and 56°C. When
performing transcription reactions at temperatures higher than the
suggested 50°C, it is recommended to limit the reaction time to 30
minutes or less as incubation of RNAs at high temperature for
prolonged periods in the presence of MgCl2 (a component of
the Hi-T7 RNA Polymerase Reaction Buffer) may lead to RNA
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.