FAQ: Does the DNA need to be purified after the dephosphorylation step and prior to the ligation step?

If you use Quick Dephosphorylation Kit (NEB #M0508), rSAP (NEB #M0371) or Antarctic Phosphatase (NEB #M0289), which can be heat inactivated, then a clean-up step is not necessary. If the alkaline phosphatase used is CIP, which cannot be heat inactivated, then a clean-up step (using Monarch® PCR & DNA Cleanup Kit NEB #T1030) is necessary.