HomeFAQsDoes the DNA need to be purified after a restriction digest and prior to the dephosphorylation step?
FAQ: Does the DNA need to be purified after a restriction digest and prior to the dephosphorylation step?
If the enzymes used in the restriction digest can be heat inactivated, then a purification step is not needed. If the enzymes used cannot be heat inactivated, then a clean-up step between the restriction digest and the dephosphorylation step is recommended.
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