HomeFAQs Which competent cell strains are compatible with Gateway® Cloning? Which strains are not compatible?
FAQ: Which competent cell strains are compatible with Gateway® Cloning? Which strains are not compatible?
NEB 5-alpha (NEB #C2987) and NEB 10-beta (NEB #C3019) Competent E. coli can be used with Gateway Cloning. Strains that contain the F´ plasmid are resistant to ccdB, and therefore cannot be used with Gateway Cloning - these include NEB Turbo (NEB #C2984), NEB 5-alpha F'Iq (NEB #C2992), and NEB Stable (NEB #C3040) Competent E.coli.
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