FAQ: Which competent cell strains are compatible with Gateway® Cloning? Which strains are not compatible?

NEB 5-alpha (NEB #C2987) and NEB 10-beta (NEB #C3019) Competent E. coli can be used with Gateway Cloning.  Strains that  contain the F´ plasmid are resistant to ccdB, and therefore cannot be used with Gateway Cloning - these include NEB Turbo (NEB #C2984), NEB 5-alpha F'Iq (NEB #C2992), and NEB Stable (NEB #C3040) Competent E.coli.