FAQ: Can the Monarch Total RNA Miniprep Kit (NEB #T2010) be used to purify RNA from saliva?

Yes. We recommend stabilizing saliva samples in Monarch DNA/RNA Protection Reagent (NEB #T2011) and processing samples as described below. It is important to note that RNA purified from saliva is typically degraded with RIN values between 2.0-3.0.

  1. Immediately stabilize 200µl of the saliva sample after collection by mixing with an equal volume of 2X Monarch DNA/RNA Protection Reagent. If unable to add the Protection reagent immediately, briefly place saliva sample on ice until Protection Reagent can be added. 
  2. For every 400 μl of DNA/RNA Protection Reagent/Sample mixture 20 μl Monarch Proteinase K. Vortex briefly and incubate at room temperature for 30 minutes.
  3. Add an equal volume of Monarch RNA Lysis Buffer (NEB #T2012) and vortex briefly. Proceed to Step 1 of Part 2: RNA Binding and Elution in the Product Manual.