FAQ: How many indices are available with NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) (NEB #E6440, E6442, E6444, and E6446)?

Each kit comprises pairs of 96 unique i5 indices and 96 unique i7 indices. Each well of the primer plate contains a unique pair of one i5 index primer and one i7 index primer, thereby providing 96 unique dual indices. When used together, all four sets total 384 indices for multiplexing.