HomeFAQsWhy are two different neuraminidase enzymes provided with the N-Glycan Sequencing Kit?
FAQ: Why are two different neuraminidase enzymes provided with the N-Glycan Sequencing Kit?
α2-3,6,8,9 Neuraminidase A (NEB# P0722) has a broader specificity, as it cleaves both α2-3,6,8,9 linked Neu5Ac (N-Acetylneuraminic acid) and Neu5Gc (N-Glycolylneuraminic acid) residues. Neu5Gc epitopes are found in murine derived antibodies. α2-3 Neuraminidase S (NEB# P0743) cleaves only α2-3 linked Neu5Ac residues.
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