FAQ: Will the blue dye in the LunaScript®  RT SuperMix interfere with detection?

The LunaScript RT Supermix contains an inert visual reference dye that gives the mix a clear blue color. This colored appearance makes it easier to track reaction setup by identifying the presence vs. absence and volume differences of cDNA reactions inside tubes, pipet tips, and downstream qPCR reactions. The blue dye has been extensively evaluated in cDNA synthesis and qPCR experiments and it has not been observed to inhibit or interfere with RNA detection.