FAQ: What qPCR reagents do you recommend for detection of cDNA products?

The cDNA products generated using the LunaScript®  RT SuperMix have been extensively evaluated in qPCR using the Luna® Universal qPCR Master Mix (NEB #M3003) and the Luna Universal Probe qPCR Master Mix (NEB #M3004). In combination, these products provide a two-step RT-qPCR workflow with excellent sensitivity and accurate, linear quantitation. In addition, the LunaScript RT SuperMix is compatible with other major commercial qPCR mixes.