FAQ: Should I include a No-Reverse Transcriptase (RT) control reaction when setting up my cDNA synthesis reaction?

Yes. When present, genomic DNA or carryover products can interfere with accurate RNA quantitation, particularly for low copy targets. Therefore, it is important to carry out the appropriate No-RT control reactions to account for these effects. In addition, no template control (NTC) reactions should be set up to demonstrate that positive reactions are meaningful.