FAQ: How much RNA template should I use in my LunaScript®  cDNA synthesis reactions?

The LunaScript RT Supermix works well with purified RNA templates including total RNA, mRNA, and in vitro transcript samples.

  Amount in 20 μl reaction
Total RNA ≤ 1 μg
Poly(A)-RNA ≤ 1 μg
In vitro transcripts 100 ng